Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi
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منابع مشابه
Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi
BACKGROUND Clustered regularly interspaced short palindromic repeats interference (CRISPRi) has provided an efficient approach for targeted gene inhibition. A non-model microorganism Halomonas species TD01 has been developed as a promising industrial producer of polyhydroxyalkanoates (PHA), a family of biodegradable polyesters accumulated by bacteria as a carbon and energy reserve compound. A c...
متن کاملEngineering bacteria for enhanced polyhydroxyalkanoates (PHA) biosynthesis
Polyhydroxyalkanoates (PHA) have been produced by some bacteria as bioplastics for many years. Yet their commercialization is still on the way. A few issues are related to the difficulty of PHA commercialization: namely, high cost and instabilities on molecular weights (Mw) and structures, thus instability on thermo-mechanical properties. The high cost is the result of complicated bioprocessing...
متن کاملEnhanced polyhydroxyalkanoates accumulation by Halomonas spp. in artificial biofilms of alginate beads.
Microbial mats are complex but stable, multi-layered and multi-functional biofilms, which are the most frequent bacterial formations in nature. The functional strategies and physiological versatility of the bacterial populations growing in microbial mats allow bacteria to resist changing conditions within their environment. One of these strategies is the accumulation of carbon- and energy-rich ...
متن کاملComparative genomics study of polyhydroxyalkanoates (PHA) and ectoine relevant genes from Halomonas sp. TD01 revealed extensive horizontal gene transfer events and co-evolutionary relationships
BACKGROUND Halophilic bacteria have shown their significance in industrial production of polyhydroxyalkanoates (PHA) and are gaining more attention for genetic engineering modification. Yet, little information on the genomics and PHA related genes from halophilic bacteria have been disclosed so far. RESULTS The draft genome of moderately halophilic bacterium, Halomonas sp. TD01, a strain of g...
متن کاملBacterial Cellulose-polyhydroxyalkanoates Composites Synthesis, physico-chemical characterization and biological evaluation for tissue engineering
CATALIN ZAHARIA1, EUGENIU VASILE1, BIANCA GALATEANU2, MIHAELA-CRISTINA BUNEA1, ANGELA CASARICA3, PAUL OCTAVIAN STANESCU1* 1University Politehnica of Bucharest, Advanced Polymers Materials Group 149 Calea Victoriei, 010072 Bucharest, Romania 2University of Bucharest, Department of Biochemistry and Molecular Biology, 34-36 M. Kogalniceanu Blv., 050107, Bucharest, Romania 3National Institute for C...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2017
ISSN: 1475-2859
DOI: 10.1186/s12934-017-0655-3